Sudan Black B is a slightly basic dye that combines with the acidic groups in the lipid compounds, hence staining the phospholipids, lipoproteins, and triglycerides found in the staining specimen.

use of Sudan Black B for staining and direct observation under the microscope enables the rapid observation of the qualitative status of lipid production in the cells.

Sudan III Stain is used to detect fat in feces, urine and tissues. Patients demonstrating fat in stool (i.e., steatorrhea) may have a correlation to pancreatic diseases or other fat absorption diseases.

SUDAN BLACK B FOR MICROSCOPY