Sodium citrate is used as anticoagulant to prevent blood from clotting. Dispense 0.5 ml of sodium citrate into test tube. Add 4.5 ml blood and mix gently by inversion of the stoppered tube. Sodium citrate is effective as an anticoagulant due to its mild calcium-chelating properties.

Sodium citrate is used as anticoagulant to prevent blood from clotting. Dispense 0.5 ml of sodium citrate into test tube. Add 4.5 ml blood and mix gently by inversion of the stoppered tube. 

Serious side effects of citric acid, potassium citrate, and sodium citrate include numbness or tingly feeling, swelling or rapid weight gain, muscle twitching or cramps, fast or slow heart rate, confusion, or mood changes, bloody or tarry stools, severe stomach pain, ongoing diarrhea, or seizure 

Here follows the procedure to prepare your anticoagulant solution: Dissolve 3.80g Sodium citrate in 100.0ml Distilled water. (Stir until solids are no longer observed.)

SODIUM CITRATE SOLUTION 3.8 %