It can be used for histopathological diagnosis of the Plasmodium species that cause malaria and some other spirochete and protozoan blood parasites. It is also used in Wolbachia cell stain in Drosophila melanogaster. Giemsa stain is a classic blood film stain for peripheral blood smears and bone marrow specimens.

Generation. Giemsa's solution is a mixture of methylene blue, eosin, and Azure B. The stain is usually prepared from commercially available Giemsa powder. A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide.

GIEMSA STAINING SOLUTION