Nuclear staining is carried out where Borax Carmine (Grenacher's) Aqueous stain is used as one of the stains and staining characteristics is observed under microscope by using oil immersion lens. Results Nuclei, zone of membranells -Deep pink, Other organelles - Deep pink, Cytoplasm - Transparent.

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With the small volume of material usually stained in protozoal work, it is easily possible to pass from basic to a strongly acid solution with the dye.

Borax Carmine Grenacher Aqueous Staining Solution